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Filtered Search Results
Thermo Scientific™ MssI (PmeI) (5 U/μL)
The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | MssI (PmeI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer B |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ FastDigest Eco91I
Cut at G^GTNACC sites with Thermo Scientific™ FastDigest™ Eco91I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ Eco47I (AvaII) (10 U/μL)
The Eco47I (AvaII) restriction enzyme recognizes G^GWCC sites and cuts best at 37°C in R buffer (Isoschizomers: AvaII, Bme18I, SinI, VpaK11BI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ PaeI (SphI) (10 U/μL)
The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PaeI (SphI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer B |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest NheI
Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nhe I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest Alw44I
Cut at G^TGCAC sites with Thermo Scientific™ FastDigest™ ApaLI (Alw44I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: VneI).
Thermo Scientific™ UTP Solution (100 mM)
Thermo Scientific UTP (uridine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ FastDigest MreI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ TaqI (10 U/μL)
The TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65°C in its own unique buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest AanI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest MssI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest SmaI
The FastDigest SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (isoschizomers: TspMI, XmaCI; neoschizomer: XmaI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Sma I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | Blunt |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ Esp3I (BsmBI) (10 U/μL)
The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Esp3I (BsmBI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Scarless Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | Yes |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest AatII
Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).
Thermo Scientific™ FastDigest BglI
Cut at GCCNNNN^NGGC sites with Thermo Scientific™ FastDigest™ BglI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.